Sub-Lethal Peak Exposure to Insecticides Triggers Olfaction-Mediated Avoidance in Zebrafish Larvae

In agricultural areas, insecticides inevitably reach water bodies via leaching or run-off. While designed to be neurotoxic insects, have adverse effects on a multitude of organisms due the high conservation nervous system among phyla. To estimate ecological insecticides, it is important investigate their impact non-target such as fish. Using zebrafish model, we investigated how different classes influence fish behavior and uncovered neuronal underpinnings associated behavioral changes, providing an unprecedented insight into perception these chemicals by We observed that larvae avoid diazinon imidacloprid while showing no response other with same mode action. Moreover, ablation olfaction abolished aversive responses, indicating smelled insecticides. Assessment activity in 289 brain regions showed hypothalamic areas involved stress were largest resembles reactions stimuli threaten homeostasis, changes chemistry. Our results contribute understanding environmental insecticide exposure can help refine acute toxicity assessment.